Universal Fiber Coupling Solution

Connect any of our steady state and hybrid fluorometers to virtually any upright or inverted microscope!

HORIBA Scientific’s con­focal, universal fiber coupling solution allows any of our steady state and hybrid fluorometers to be easily connect­ed to virtually any upright or inverted microscope. Spectrofluorometric capabilities include the unparalleled precision and sensitivity of Single-Photon Counting and the speed and sensitivity of CCD detection. Similarly, fluorescence spectral mapping capabilities utilize an automated fast scanning X-Y stage to generate rapid spectral maps with micron level spatial resolution. Of course our microscope solutions preserve the microscope's native optical performance and capabilities. For example, they add no optics to the entire emission optical path. Use the high performance cooled fluorescence camera to image your field of interest. Once you identify a region to spectrally analyze, simply switch your filter cube to a beam splitter or an appropriate dichroic, divert the light to the fluorometer, and you are collecting high resolution spectra. Custom solutions are also available to measure in the UV and NIR regions, beyond the normal range of your microscope.

 

 

Segment: Scientific
Division: Fluorescence
Manufacturing Company: HORIBA Scientific

Couple a microscope and:

  • Measure complete spectra of samples as small as 1 μm.
  •  Get spectra from as little as a few molecules of sample.
  •  Perform localized FRET measurements.

Add an automated stage and camera, and:

  • Create complete spatial/spectral maps.
  • Perform repetitive QC characterization of structured samples like photovoltaics. 
SpecificationComplete Microscope SolutionInterface to Existing Microscope
ObjectivePlan achromat x10 and x60, other magnifications available. 6 position turretUser supplied
Confocal pinholesKinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mmKinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mm
Dimensions, Weight68 cm x 46 cm x 60 cm, 40 kg (BX53)              
90 cm x 50 cm x 75 cm, 65 kg (IX73)
 
Excitation source: Scopelite 200 (Optional)
Wavelength rangeFrom 350 to 700 nmFrom 350 to 700 nm
Illumination Adjustment5 preset % apertures: 0, 25, 50, 75, 1005 preset % Apertures: 0, 25, 50, 75, 100
Output Power7 Watts7 Watts
Lamp Life2000 hrs2000 hrs
Warm Up2 min2 min
Long Term Drift<0.1% RMS<0.1% RMS
CoolingAir cooled by fanAir cooled by fan
Dimensions20 x 14.5 x 25.4 cm (WxDxH)20 x 14.5 x 25.4 cm (WxDxH)
Weight3.4 kg3.4 kg
Filters
ND filter sliders6 positions: 0, 0.3, 0.6, 1, 2 and 3 ODUser supplied
Fibers
Multimode slit to roundUV-VIS : 1.5 m length, 250-850 nm wavelength range
NIR : 1.5 m length, 350-2400 nm wavelength range
UV-VIS : 1.5 m length, 250-850 nm wavelength range
NIR : 1.5 m length, 350-2400 nm wavelength range
Fiber Coupling
ExcitationCollimating lens adapters for OlympusCollimating Lens Adapters for Olympus, Nikon, Leica and Zeiss
EmissionUniversal C-mount adapter with pinhole holderUniversal C-mount adapter with pinhole holder
Camera    Required for mapping optionRequired for mapping option
Fluorescence camera12 bits, 1.4 Mpix, cooled, low noise12 bits, 1.4 Mpix, cooled, low noise
Motorized stageRequired for mapping optionRequired for mapping option
Repeatibility
Travel range
Manual control
Automatic control
<1.0 µm
75 x 50 mm
With joystick
Through FluorEssence software
Suitable stage


Through FluorEssence software
Software/Computer
FluorEssence
PC requirements
Windows® 7, 32- or 64-bit
At least three available USB ports
2 GB RAM
2 GB hard-disk space
DVD-ROM drive
Video resolution of at least 1280x800
Windows® 7, 32- or 64-bit
At least three available USB ports
2 GB RAM
2 GB hard-disk space
DVD-ROM drive
Video resolution of at least 1280x800
 

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