Duetta

Duetta Fluorescence and Absorbance spectrometer

Fluorescence and Absorbance Spectrometer

From the global leaders in fluorescence comes a new bench-top instrument and a new concept for analytical molecular spectroscopy.

Duetta- Fluorescence and Absorbance Spectrometer Promo Video (1:02)

Duetta- Fluorescence and Absorbance Spectrometer Promo Video

Not Your Standard Spectrometer Video (4:29)

Not Your Standard Spectrometer, Duetta 4 Awards Video

Duetta-Fluorescence and Absorbance Spectrometer at the 2018 Biophysical Society Meeting Video (5:03)

Duetta-Fluorescence and Absorbance Spectrometer at the 2018 Biophysical Society Meeting

EzSpec™ Fluorescence and Absorbance Software Video (2:19)

EzSpec™ Fluorescence and Absorbance Software

 

Segment: Scientific
Division: Fluorescence
Manufacturing Company: HORIBA Scientific

A Superior Spectrometer Concept

The Future of Fluorescence

  • Two-in-one Fluorescence and Absorbance Spectrometer
  • UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm
  • Full 3-D Fluorescence EEM Acquisition in Less Than One Second
  • Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman
  • Automatic Correction for Primary and Secondary Inner Filter Effects (IFE)
  • High Fidelity Molecular Fingerprinting with Unique A-TEEM™(Absorbance-Transmittance Excitation Emission Matrix) Technology
  • Millisecond CCD Detection of Entire Fluorescence Spectrum

Fluorescence and Absorbance in one Spectrometer!

Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.

Fluorescence in the Blink of an Eye!

Duetta’s ultra-fast, state-of-the-art CCD technology allows it to outperform, by orders of magnitude, any competitor using PMTs. It’s the fastest bench-top fluorometer in the world, and the only fluorometer that can acquire a full spectrum in less than 100 milliseconds! This state-of-the-art CCD technology also extends NIR wavelength detection to 1,100 nm, well beyond the limit of a standard PMT fluorometer.

Highest Fluorescence Sensitivity in its Class

Having the highest level of fluorescence sensitivity means that Duetta’s optimized design lets you measure lower concentrations of a sample, and provides better quality data.

EzSpec™ Touch-Screen Software!

Duetta debuts HORIBA’s new generation software interface with touch-screen utility, and a selection of Apps for routine analysis.

A New Spectrometer Design

Fluorescence and Absorbance

Duetta’s two specialized sample detectors collect fluorescence at right angles to the excitation light, and absorbance straight through the sample.

Absolute Fluorescence Spectra with Real Time Inner Filter Effect (IFE) Correction

IFE limits the linearity of the fluorescence signal at higher sample concentrations due to primary and secondary re-absorption of fluorescence. Duetta’s unique design applies real time IFE correction to obtain absolute fluorescence spectra at higher sample concentrations.

CCDs Collect the Entire Emission Spectrum

Ergonomic, Hands Free, Sample Access

Duetta’s CCD detection takes a snapshot of the entire fluorescence emission spectrum from 250 to 1,100 nm. (So not only is it much faster but it also gives you much more information than the competition.)

Extended NIR Wavelength Range up to 1,100 nm

Near Infrared applications are growing rapidly, and Duetta is ready to meet the challenge with fluorescence detection from 250 to 1,100 nm, well beyond the reach of standard PMTs.

Emission from Neodymium Glass
NIR detection comparing Duetta with PMT.

Easy Lamp Cartridge Replacement Lamp

Lamp cartridges are pre-aligned at the factory, guaranteeing perfect alignment upon snap-in replacement. No tools or service are required.

Smart Sample Accessory Trays

Switching from one type of sample holder to another could not be easier.

Without any tools or cables, Duetta’s spill-proof sample accessory trays are identified by EzSpec software, enable electrical power for the appropriate module (e.g. 4-position Peltier holder), and read an enabled
sensor (e.g. temperature), if available.

Fully Automated Order Sorting Filters

Duetta includes excitation and emission cut off filters for automated elimination of second order grating effects, which otherwise interfere with the fluorescence signal of interest.

EEMs Acquired in Seconds
Excitation Emission Matrix (EEM) acquired in 1 second, shows no second order scattering.

EzSpec Software

Apps at your Fingertips

From the EzSpec launch screen, each Apps icon intuitively helps you see and select the analysis you want to perform with a simple point and touch.

Apps:

  • Autonomous Acquisition
  • Pass/Fail
  • Build/Use a Concentration Curve
  • Protein A280

 

 

EzSpec lets you see your recent method and data, and your personal favorites.

Widest Range of Spectroscopic Acquisition Modes:

  • Fluorescence emission spectra plus Abs & IFE*
  • Fluorescence excitation spectra plus Abs & IFE*
  • Capture fluorescence value plus Abs & IFE*
  • Fluorescence EEM plus Abs & IFE*
  • Fluorescence kinetics (emission spectra* or single point intensities)
  • Absorbance and % Transmittance (spectra or kinetics)

 

* Unique to Duetta

Acquire Menu

PDF Report Generator or Export:

  • Includes data and results of fitting/processing
  • Full method details
  • 2D and 3D graphs
  • Instrument and user identification information
  • Customizable logo
  • Optional and customizable signature fields
  • Full data and method traceability

Accessories

Optional Sample Holder Trays

SampleSnap-WJ: Thermostated and Micro-Stirred Cuvette Sample Tray

Self-identifying thermostatable, water jacketed cuvette holder (1 cm) with magnetic microstir motor. Sample tray can be installed in seconds without the need of a tool, and provides power for micro-stirrer. Requires, but does not include, external water bath.

SampleSnap-1Pelt: Single Cuvette Peltier Holder Sample Tray

Self-identifying single cuvette Peltier holder (1 cm) for automated temperature control and ramping from -20 to +105 degrees Celsius, with magnetic micro-stirrer. Sample tray can be installed in seconds without the need of a tool, and provides power and control of the Peltier device, and reads temperature. Includes external water circulator for heat sink.

SampleSnap-4Pelt: Four Position Peltier Cuvette Sample Tray

Self-identifying four position Peltier holder (1 cm) for automated temperature control and ramping from -40 to +105 degrees Celsius, with magnetic micro-stirrers under all four positions. Sample tray can be installed in seconds without the need of a tool, and provides power and control of the Peltier device, and reads temperature. Includes external water circulator for heat sink.

SampleSnap-UNI: Universal Variable Angle Sample Tray

Self-identifying sample holder with 360 degree variable angle goniometer for empirical determination of optimum sample angle. Sample tray can be installed in seconds without need of a tool. Requires, but does not include, solid sample holder accessory (SampleSnap-SS), powder sample holder accessory (SampleSnap-PWD) or cuvette holder accessory (SampleSnap-VACVT).

Sample Accessories for SampleSnap-UNI Universal Variable Angle Sample Tray

  • SampleSnap-SS: Solid Sample Accessory
    Solid sample accessory for universal sample holder to be used with flat or solid samples such as thin films, cover slips, paper, gemstones and more. Requires, but does not include, SampleSnap-UNI.
  • SampleSnap-PWD: Powder Sample Accessory
    Powder sample accessory for universal sample holder. Requires, but does not include, SampleSnap-UNI.
  • SampleSnap-VACVT: Variable Angle Cuvette Sample Accessory
    Cuvette sample accessory for universal sample holder. Requires, but does not include, SampleSnap-UNI.

Additional Accessories

K-210: Six pack of windows for powdered sample holder
K-151: 10 mm quartz cuvette
LampSnap: Replacement Duetta light source assembly with pre-aligned xenon arc lamp
K-AFILTER: Replacement Duetta air intake filter

* Duetta includes a standard room temperature single cuvette sample holder tray.

Applications

Duetta supports applications in a wide variety of fields

  • Chemistry
  • Life Sciences
  • Materials Research
  • Food Science
  • Earth Sciences

Chemistry/Photochemistry

Pyrene in a solution of A-B-A triblock copolymer micelles is solvated in the hydrophobic core solvent environment. 3D EEMs can be shown in contour, waterfall plot, or 2D overlay mode.

Biology and Biochemistry

BSA protein measured from 5° C to 60° C and the peak intensity and peak wavelength are plotted vs. temperature of the protein solution. Measured using the SampleSnap-4Pelt 4-position Peltier temperature-controlled cuvette holder.

Materials

Polymer Aggregation and Structure. Temperature dependent spectra showing polymer micellization.

Molecular Binding (FRET)

A. Kinetic spectral scans of native BSA protein with rapidly added ANS (3x10-6 M). Spectra taken every 100 ms after ANS addition. As ANS binds to native BSA, the BSA Trp emission decreases and the ANS emission increases as a result of FRET due to proximity of excited Trp and ANS. 
B. Kinetic spectral scans of SDS-denatured BSA after addition of ANS. Much higher ANS concentration (4x10-5 M) is required to affect binding to enatured BSA; no FRET is observed due to increased distances between ANS and Trp.

Rare Earths

Emission spectra of a series of glass materials containing different lanthanides. Measured using the SampleSnap-Uni solid sample holder.

Protein Biophysics

Comparison of bovine serum albumin protein and the same protein denatured by sodium dodecylsulfate (SDS). The emission peak narrows and shifts 15 nm to the blue.

Pharmaceuticals

Quenching: Fluorescence monitoring of the titration of 1 M potassium iodide (KI) into a solution of 2-aminopyridine, a common small molecule drug pre-cursor.

 

 DuettaDuetta-Bio
Acquisition ModesAbsorbance and Fluorescence Spectrometer
Fluorescence SensitivityWater Raman SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits
Spectral Acquisition Rate510,000 nm/min
EEM Acquisition RateAs fast as 1 second (sample and wavelength dependent)
A-TEEM Acquisition RateAs fast as 30 seconds (sample and wavelength dependent)
Fluorescence DetectorCCD/Spectrograph
Fluorescence Detector Range250 to 1,100 nm
Fluorescence Bandwidth1, 2, 3, 5,10, 20 nm (excitation and emission)
Light Source75 W Xenon arc lamp. Dedicated cartidge for snap-in replacement
Excitation/Absorbance Wavelength Range250 to 1,000 nm
Absorbance DetectorSilicon Photodiode
Absorbance Detector Range250 to 1,000 nm
Absorbance Bandwidth1, 2, 3, 5,10, 20 nm
Absorbance Range0 to 2 A
Absorbance Accuracy+/- 0.02 A
Wavelength Accuracy+/- 1 nm
Computer RequirementsWindows® 7/8/10, 64-bit OS, laptop or PC with at least 1 USB (more for additional accessories)
SoftwareEZSpec
Dimensions (W x D x H)17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm
Weight45 pounds; 20.4 kg
Polarizers (Time of Purchase)Not AvailableYes (280 to 750 nm)
Quantum Dot Absorbance, Photoluminescence Spectra and Lifetimes
Quantum Dot Absorbance, Photoluminescence Spectra and Lifetimes
Quantum dots (QDs) are semiconducting spheres in the size typically in the range of 1 to 10nm. The size of these small spheres give quantum dots the semiconducting properties and resulting photoluminescence that would not necessarily occur for the same material on larger scales.
Protein A280 for Protein Concentration
Protein A280 for Protein Concentration
The new HORIBA Duetta fluorescence and absorbance spectrometer offers many unique benefits for molecular spectroscopy. It is primarily thought of as a spectrofluorometer that combines absorbance and fluorescence spectroscopy to correct the fluorescence fingerprint for concentration-related effects. Duetta can also be used as a precise absorbance spectrometer. This application note explores the use of Duetta for a common absorbance application, the Protein A280 application.

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