Unlocking Innovation Across Disciplines
Peer-reviewed publications worldwide showcase the role of HORIBA Duetta’s dual fluorescence and absorbance spectrometer in various fields, including environmental monitoring, nanotechnology, biomedical breakthroughs, and advanced materials science. Its unique combination of high-speed, sensitive fluorescence and absorbance measurements, advanced spectral correction, and multivariate analysis capabilities has made it a go-to instrument for researchers in dozens of fields.
"EEM spectroscopy can be implemented as a powerful technique for determining the purity of complex mixtures, especially when other techniques, including mass spectrometry, fail to provide adequate characterization of a given material."... "The determination of different cluster species was only made possible with the complete fluorescence profiles provided from EEM spectroscopy."
- K. Stamplecoskie et al. "The power of fluorescence excitation–emission matrix (EEM) spectroscopy in the identification and characterization of complex mixtures of fluorescent silver clusters"
“We endeavored to take as much advantage as possible of the wide excitation and emission capability of the HORIBA instrument (excitation/ emission matrix, EEM) so that emission at unexpected wavelengths was not missed.”
- S. Sma-Air et al, Spectrofluorometric insights into the application of PAM fluorometry in photosynthetic research
"EEM spectroscopy can be implemented as a powerful technique for determining the purity of complex mixtures, especially when other techniques, including mass spectrometry, fail to provide adequate characterization of a given material."... "The determination of different cluster species was only made possible with the complete fluorescence profiles provided from EEM spectroscopy."
- K. Stamplecoskie et al. "The power of fluorescence excitation–emission matrix (EEM) spectroscopy in the identification and characterization of complex mixtures of fluorescent silver clusters""The inherent value of EEMS is not just for characterizing a single emission species!”
- H. Ramsay et al. "The power of fluorescence excitation-emission matrix (EEM) spectroscopy in the identification and characterization of complex mixtures fluorescent silver clusters.”"EEM provided a thorough evaluation of the correlations between the excitation and emission spectra."
- S. Takahama et al, “Variation in the responses of carbon quantum dots (CQDs) synthesized from native coconut husk and coconut husk-derived charcoal.”
“Using EEM spectroscopy, it is possible to visualize the complete fluorescence profile of each of the three cluster species isolated from RP-HPLC."
- H. Ramsey et al, "The power of fluorescence excitation-emission matrix (EEM) spectroscopy in the identification and characterization of complex mixtures fluorescent silver clusters."
"The Duetta can measure absorbance and fluorescence simultaneously allowing easy inner filter correction. All spectra were corrected for both Rayleigh scattering and inner filter effects."
- AW Harrison et al, “Reaction of Glyoxal and Ammonium as a Potential Contributor to Protein-like Fluorescence in Atmospheric Measurements.”
"EEM was an efficient method for determining the optical properties of CQDs in detail."
- S. Takahama et al, “Variation in the responses of carbon quantum dots (CQDs) synthesized from native coconut husk and coconut husk-derived charcoal.”
Explore the Evidence
Duetta’s impact is documented in leading journals such as Nature Chemistry, Journal of the American Chemical Society, ACS Sustainable Chemistry & Engineering, Bioactive Materials, Food Chemistry, and many more. Researchers worldwide—from the UK to China, the USA to Saudi Arabia—trust Duetta for applications as varied as live-cell imaging, environmental remediation, nanomedicine, and industrial process optimization.
The Future of Fluorescence
Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.
Duetta’s ultra-fast, state-of-the-art CCD technology allows it to outperform, by orders of magnitude, any competitor using PMTs. It’s the fastest bench-top fluorometer in the world, and the only fluorometer that can acquire a full spectrum in less than 100 milliseconds! This state-of-the-art CCD technology also extends NIR wavelength detection to 1,100 nm, well beyond the limit of a standard PMT fluorometer.
Having the highest level of fluorescence sensitivity means that Duetta’s optimized design lets you measure lower concentrations of a sample, and provides better quality data.
Duetta debuts HORIBA’s new generation software interface with touch-screen utility, and a selection of Apps for routine analysis.
Watch how EzSpec software performs the Pass/Fail |
EzSpec Software obtains a concentration curve |
EzSpec Software executes a Protein A280 concentration |
EzSpec Software discovers Fluorescence profiles in about 60s |
Duetta’s two specialized sample detectors collect fluorescence at right angles to the excitation light, and absorbance straight through the sample.
IFE limits the linearity of the fluorescence signal at higher sample concentrations due to primary and secondary re-absorption of fluorescence. Duetta’s unique design applies real time IFE correction to obtain absolute fluorescence spectra at higher sample concentrations.
Ergonomic, Hands Free, Sample Access
Duetta’s CCD detection takes a snapshot of the entire fluorescence emission spectrum from 250 to 1,100 nm. (So not only is it much faster but it also gives you much more information than the competition.)
Near Infrared applications are growing rapidly, and Duetta is ready to meet the challenge with fluorescence detection from 250 to 1,100 nm, well beyond the reach of standard PMTs.
Emission from Neodymium Glass
NIR detection comparing Duetta with PMT.
Lamp cartridges are pre-aligned at the factory, guaranteeing perfect alignment upon snap-in replacement. No tools or service are required.
Switching from one type of sample holder to another could not be easier.
Without any tools or cables, Duetta’s spill-proof sample accessory trays are identified by EzSpec software, enable electrical power for the appropriate module (e.g. 4-position Peltier holder), and read an enabled
sensor (e.g. temperature), if available.
Duetta includes excitation and emission cut off filters for automated elimination of second order grating effects, which otherwise interfere with the fluorescence signal of interest.
EEMs Acquired in Seconds
Excitation Emission Matrix (EEM) acquired in 1 second, shows no second order scattering.
From the EzSpec launch screen, each Apps icon intuitively helps you see and select the analysis you want to perform with a simple point and touch.
Apps:
EzSpec lets you see your recent method and data, and your personal favorites.
* Unique to Duetta
Self-identifying thermostatable, water jacketed cuvette holder (1 cm) with magnetic microstir motor. Sample tray can be installed in seconds without the need of a tool, and provides power for micro-stirrer. Requires, but does not include, external water bath.
Self-identifying single cuvette Peltier holder (1 cm) for automated temperature control and ramping from -15 to +105 degrees Celsius, with magnetic micro-stirrer. Sample tray can be installed in seconds without the need of a tool, and provides power and control of the Peltier device, and reads temperature. Includes external water circulator for heat sink.
Self-identifying four position Peltier holder (1 cm) for automated temperature control and ramping from -15 to +110 degrees Celsius, with magnetic micro-stirrers under all four positions. Sample tray can be installed in seconds without the need of a tool, and provides power and control of the Peltier device, and reads temperature. Includes external water circulator for heat sink.
Self-identifying sample holder with 360 degree variable angle goniometer for empirical determination of optimum sample angle. Sample tray can be installed in seconds without need of a tool. Requires, but does not include, solid sample holder accessory (SampleSnap-SS), powder sample holder accessory (SampleSnap-PWD) or cuvette holder accessory (SampleSnap-VACVT).
K-210: Six pack of windows for powdered sample holder
K-151: 10 mm quartz cuvette
LampSnap: Replacement Duetta light source assembly with pre-aligned xenon arc lamp
K-AFILTER: Replacement Duetta air intake filter
* Duetta includes a standard room temperature single cuvette sample holder tray.
Duetta supports applications in a wide variety of fields
Pyrene in a solution of A-B-A triblock copolymer micelles is solvated in the hydrophobic core solvent environment. 3D EEMs can be shown in contour, waterfall plot, or 2D overlay mode.
BSA protein measured from 5° C to 60° C and the peak intensity and peak wavelength are plotted vs. temperature of the protein solution. Measured using the SampleSnap-4Pelt 4-position Peltier temperature-controlled cuvette holder.
Polymer Aggregation and Structure. Temperature dependent spectra showing polymer micellization.
A. Kinetic spectral scans of native BSA protein with rapidly added ANS (3x10-6 M). Spectra taken every 100 ms after ANS addition. As ANS binds to native BSA, the BSA Trp emission decreases and the ANS emission increases as a result of FRET due to proximity of excited Trp and ANS.
B. Kinetic spectral scans of SDS-denatured BSA after addition of ANS. Much higher ANS concentration (4x10-5 M) is required to affect binding to enatured BSA; no FRET is observed due to increased distances between ANS and Trp.
Emission spectra of a series of glass materials containing different lanthanides. Measured using the SampleSnap-Uni solid sample holder.
Comparison of bovine serum albumin protein and the same protein denatured by sodium dodecylsulfate (SDS). The emission peak narrows and shifts 15 nm to the blue.
Quenching: Fluorescence monitoring of the titration of 1 M potassium iodide (KI) into a solution of 2-aminopyridine, a common small molecule drug pre-cursor.
| Duetta | Duetta-Bio | |
| Acquisition Modes | Absorbance and Fluorescence Spectrometer | |
| Fluorescence Sensitivity | Water Raman SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits | |
| Spectral Acquisition Rate | 510,000 nm/min | |
| EEM Acquisition Rate | As fast as 1 second (sample and wavelength dependent) | |
| A-TEEM Acquisition Rate | As fast as 30 seconds (sample and wavelength dependent) | |
| Fluorescence Detector | CCD/Spectrograph | |
| Fluorescence Detector Range | 250 to 1,100 nm | |
| Fluorescence Bandwidth | 1, 2, 3, 5,10, 20 nm (excitation and emission) | |
| Light Source | 75 W Xenon arc lamp. Dedicated cartidge for snap-in replacement | |
| Excitation/Absorbance Wavelength Range | 250 to 1,000 nm | |
| Absorbance Detector | Silicon Photodiode | |
| Absorbance Detector Range | 250 to 1,000 nm | |
| Absorbance Bandwidth | 1, 2, 3, 5,10, 20 nm | |
| Absorbance Range | 0 to 2 A | |
| Absorbance Accuracy | +/- 0.02 A | |
| Wavelength Accuracy | +/- 1 nm | |
| Computer Requirements | Windows 10 or 11 64-bit OS, laptop or PC with at least 1 USB port (more for additional accessories) | |
| Software | EzSpec™ | |
| Dimensions (W x D x H) | 17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm | |
| Weight | 45 pounds; 20.4 kg | |
| Polarizers (Time of Purchase) | Not Available | Yes (280 to 750 nm) |
Do you have any questions or requests? Use this form to contact our specialists.
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