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XploRA INV

Inverted Raman Microscope

The XploRA INV inverted Raman microscope combines the automation features and small footprint of the standard XploRA™ Raman microscope with the unique sampling capabilities of an inverted microscope, especially important for demanding biological applications.
 

Segment: Scientific
Manufacturing Company: HORIBA France SAS

The XploRA INV combines the exclusive automation features and small footprint of the standard XploRA™ Raman microscope with the unique sampling capabilities of an inverted microscope, especially important for demanding biological applications. These applications include:

  • cell research
  • disease detection
  • characterisation of drug-cell interactions
  • pharmaceutical verification of intercellular activities

 

In addition, the inverted geometry allows easy incorporation of AFM units for combined Raman-AFM and TERS (Tip Enhanced Raman Spectroscopy) for highest spatial resolution sample visualisation.

The open structure of the XploRA INV also permits the use of virtually all options and add-ons for inverted microscopes, including micro manipulators and “optical tweezers”, specific enclosures for cell applications, etc.

The XploRA INV also offers the option to integrate the unique scanning capabilities of HORIBA’s patented DuoScan for rapid spectroscopic multichannel Raman imaging, as well as the new 3D FCI (Fast Confocal Imaging) module, which allows ultra-fast fluorescence imaging for visualisation and verification.

    

White light image (left), fluorescence FCI image (center) and hyperspectral fluorescence image (right) images of a bovine embryo after staining with Nile Red, acquired on the XploRA INV.  The hyperspectral image illustrates the stain in different polarity states.  Data courtesy of Prof. Igor Chourpa, Université de Tours, France.

 

 

Faster Raman                                                      YES with XY stage                                                                                                           
SWIFT™ Imaging                                         Optional DuoScan™ imaging           
/ SWIFTXS (with
EMCCD)

 

Confocal Imaging                                                 0.5 μm XY                                                                                                         
                                                                              
 

Routine operation                                              Methods and scripts 
Automation

 

Full Microscope                                                  Inverted

 

 Resolution                                                         Standard + High
                                                                          

 Multi-laser                                                       532, 638, 785 nm
  Options                                                          others on request
                                                                     

Assessing Biotherapeutics Stability using Raman Spectroscopy
Assessing Biotherapeutics Stability using Raman Spectroscopy
We have measured solutions of lysozyme under conditions known to effect its physical state in order to investigate the potential of Raman spectroscopy as a non-invasive and label-free tool to assess protein formulation stability. Results from this study identified specific Raman signature bands in this protein that can be used to identify individual amino acid residues that are reflect structural changes in proteins.

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