A Simple, Fast, “Column Free” Molecular Fingerprinting Technology
A-TEEM™ Molecular Fingerprinting is a new optical technique that is ideal for comprehensive component analysis in a variety QC/QA applications, from water to wine to pharmaceuticals and more. It is a simple, fast, “column free” spectroscopic technique that simultaneously measures the absorbance, transmission and fluorescence of samples in solution, and offers unique benefits over traditional analytical techniques, such as chromatography, mass spec, IR, etc.. Fundamentally, this technique is best applied to samples in solution that have fluorescing components, like an aromatic molecule, although it also detects non-fluorescing species via absorbance. The fluorescence EEM fingerprint technique has also been applied to solids and skin. Fluorescence is a highly sensitive technique compared to other spectroscopic techniques such as absorbance, FTIR and Raman, and the three-dimensional nature of the data provides for a better foundation for complex component analysis.
A-TEEM spectroscopy refers to the ability to simultaneously acquire Absorbance, Transmittance and a fluorescence Excitation Emission Matrix (A-TEEM) of a particular sample. HORIBA pioneered this technique with the patented Aqualog system, which combines A-TEEM spectroscopy with multichannel CCD detection to provide extremely fast results.
The A-TEEM technique requires no sample preparation, beyond possibly a simple dilution, and the cost and ease of use of an A-TEEM spectrometer allows it to be used both in the research lab as well as at-line or in-line in a production process. It’s a simpler, faster, and cheaper column-free chromatography technique for fluorescence components in solution. A-TEEM has taken fluorescence spectroscopy beyond the research lab and into the analytical industrial QC/QA environment.
HORIBA A-TEEM spectrometers can be used for fluorescence EEMs and for absorbance measurements for multi-component analysis and for transmission measurements for CIE colorimetry.
ASTM D8431 Standard Test Method for Detection of Water-soluble Petroleum Oils (BTEX) by A-TEEM Optical Spectroscopy and Multivariate Analysis.
A-TEEM™ Molecular Fingerprinting Video |
Aqualog Data Stream and Sipper Video |
Spectroscopy in :60 - FAST -01 Autosampler |
EEM Acquisition Speed Comparison Video |
HORIBA Instruments is pleased to introduce a new software tool designed to automate multivariate and machine learning analysis workflows for industrial QC/QA applications of HORIBA’s patented Aqualog Absorbance- Transmission fluorescence Excitation Emission Matrix (A-TEEM™) spectrometer.
Key Applications of HMMP Tool
Key Features and Benefits
A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!
All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.
Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.
New for our Aqualog® A-TEEM™ spectrometer, the Aqualog automatic sipper accessory handles sampling from a single source, in addition to dispensing rinsing solutions, detergents and controlling reverse-flow drainage The 4-sample changer unitconnects to the main sipper unit to enable sampling of up to 4 sources.
The sipper offers convenient installation and operation, with built-in automatic cleaning, leak detection and protection. It is fully integrated into the new Aqualog 4.0 software for batch analysis and has a variety of uses in water, pharmaceutical, beverage phenolics and many other applications.
When used at a water treatment plant, the sipper and four channel accessory enable the Aqualog to automatically extract and monitor raw, settled and finished water samples. Each sample changer unit is compatible with overflow and filtration devices, serving up to 4 independent water treatment plant sources..
The Fast-01 can be configured to use a variety of sample-vials and racks to meet your application needs and enables complete temperature control. Sample vial repeats and injection volumes are easily facilitated with the Aqualog 4.2+ software, which also offers preconfigured blank files.
All data files can be exported with ISO-formatted time-date stamping and user-configurable Sample ID and repeat codes.
All aspects of the Fast-01 hardware control are at your fingertips, with key real-time access features to facilitate the configuration and execution of your batch experiments, as well as priming, cleaning and maintenance.
Fluorescence Hardware | |
|---|---|
Light source | Extended-UV: 150W vertically mounted xenon arc lamp |
Excitation range | 200 nm to upper limit of emission detector |
Excitation bandpass | 5 nm |
Excitation monochromator | Subtractive double monochromator |
Excitation gratings | 1200 gr/mm, 250 nm blaze |
Excitation wavelength accuracy | ±1 nm |
Choice of Detector | Red-extended |
Emission range | 250-800 nm |
Emission grating | 285 gr/mm; 350 nm blaze |
Hardware pixel binning | 0.58, 1.16, 2.32, 4.64 nm/pixel |
Emission bandpass | 5 nm |
Emission spectrograph | Fixed, aberration-corrected 140 mm focal length |
Emission detector | TE-cooled back-illuminated CCD |
Emission integration time | 5 ms minimum |
CCD gain options | 2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain |
Sensitivity | Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration) |
Weight | 32.72 kg (72 lbs) |
Dimensions | LWH (618 x 435 x 336 mm); (24" x 17" x 13") |
Absorbance Hardware | |
|---|---|
Scanning range | 200-800 nm (UV lamp) |
Bandpass | 5 nm |
Slew speed | Maximum 500 nm/s |
| Optical system | Corrected single-beam |
| Detector | Si photodiode |
Wavelength accuracy | +/- 1 nm |
Wavelength repeatability | +/- 0.5 |
Photometric accuracy | +/- 0.01 AU from 0 to 2 AU |
| Photometric stability | <0.002 AU per hour |
| Photometric repeatability | +/- 0.002 AU (0 to 1 AU) |
Stray light | <1% measure with Kl standard |
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