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Measuring Size of Light Absorbing Materials with Dynamic Light Scattering

Particle Characterization

Samples that absorb certain light wavelengths can pose problems with measurement on dynamic light scattering systems, as the technique relies on the laser signal being scattered by the nanoparticles being measured, rather than absorbed. Nanogold is an example of a sample that can be tricky to measure due to the surface plasmon resonance effect that leads to stronger than normal absorbance, drastically reducing the scattering signal.

In this application note, a ~26 nm nanogold sample was measured with two different setups of detector angle and detector position using the HORIBA SZ-100V2 nanoparticle analyzer. One setup was with the 90° detector and cell center position, and the other setup was with the 173° detector and cell wall position. The absorption spectrum of the nanogold was also confirmed using a HORIBA Duetta Fluorescence and Absorbance Spectrometer.

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Measuring Size of Light Absorbing Materials with Dynamic Light Scattering
In this application note, a ~26 nm nanogold sample was measured with two different setups of detector angle and detector position using the SZ-100V2 nanoparticle analyzer. One setup was with the 90° detector and cell center position, and the other setup was with the 173° detector and cell wall position. The absorption spectrum of the nanogold was also confirmed using a Duetta Fluorescence and Absorbance Spectrometer.
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