荧光光谱

显微荧光

荧光微区测试和成像

显微荧光测试方法结合了显微镜的空间分辨率与荧光光谱法的分析能力。与传统的荧光显微镜在一个或几个波长波段提供图像不同,我们的显微荧光测量可以获得图像任何一点完整的稳态光谱或寿命

我们的显微荧光系统可以基于您的显微镜,也可提供完整的显微荧光测试系统。显微荧光可完成某个点的光谱和寿命采集,或者通过精密显微镜台对样品进行x和y方向扫描,完成某个区域内每个点光谱或寿命的采集。

显微荧光测试方法可用于细胞内和细胞间生物化学研究、PV效率分析、地质样品的晶体结构研究、纳米技术和纳米粒子研究、材料科学研究、纺织品研究、药品质量控制等领域,甚至可检测假钞上的异常特征。

FLIM Microscope Solutions

Modular Solutions

Confocal Laser Scanning FLIM

InverTau™ is our new confocal laser scanning FLIM platform. InverTau is fully software controlled and designed to fit on the side port of an inverted fluorescence microscope. You can build your own microscope system around the InverTau platform, or buy a complete microscope system from HORIBA. InverTau can be enhanced to acquire real-time wide field video rate FLIM up to 30 frames per second with the addition of FLIMera. Versatile solutions are always available with local support.

  • Affordable laser scanning unit for FLIM studies
  • Fully computer-controlled confocal optics, with the intuitive EzTime™ Image software, minimizes set up time and increases productivity
  • Simply switch from confocal laser scanning to widefield imaging with the addition of FLIMera
  • Real time video display up to 6 fps with InverTau, and up to 30 fps with FLIMera
  • Intuitive software and automated optics minimize setup time and increase productivity

Wide Field Video Rate FLIM Dynamics

The HORIBA FLIMera camera is a new concept in FLIM technology. It is a wide field imaging camera, rather than a confocal point scanning system, with the intrinsic benefit of being able to study FLIM dynamics at video rates with a SPAD array camera technology that is very simple to implement. Each of the 24,576 pixels has its own TCSPC architecture, acquiring simultaneous decays for all pixels at up to 30 frames per second.

  • Fluorescence Lifetime Mapping (FLIM)

Steady State and Time Resolved Modular Fluorometer Microscopes

HORIBA fluorescence spectroscopy solutions can be added to an inverted or upright fluorescence microscope. Many of the steady state and time-resolved experiments that are performed macroscopically in our various fluorometers, can also be performed on the microscopic scale with the addition of the appropriate optical coupling to a fluorescence microscope.

  • Nanolog: Single point UV-Vis-NIR spectra and decays, plus spectral mapping with CCD
  • Fluoromax Plus: Single point UV-Vis spectra and decays, plus spectral mapping
  • Fluorolog-QM: Single point UV-Vis-NIR spectra and decays

Fluorescence microfluorimetry is used for intra- and intercellular biochemistry, PV efficiency analysis, crystal structure of geological specimens, nanotechnology and nanoparticles, materials science, textiles, quality-control of pharmaceuticals, even detection of anomalous features on counterfeit banknotes.

A-TEEM Spectroscopy

A-TEEM Spectroscopy

Science in Action

Spectroscopy Matters

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