表面等离子体共振(SPRi)

Surface Chemistry

The SPRi-Biochips™ are suitable for a range of surface chemistries and are not restricted to a single probe/target regime. The biochips are easily adapted for specific processes.

We can offer you:

Pre-functionalized surfaces - ready-to-use
Electroaddressing of your molecules onto the biochip - we do it for you
Training on our surface chemistry
Bare gold surface

List of SPRi-Biochips and SPRi-Slides available appropriate to the probe molecules you want to immobilize

	Direct Coupling						Electro-addressing
	CS	CO	CPe	CSe	COe	CTg	CPY	CDZ
Nucleic Acids			X				X	X
Proteins	X	X	X	X	X		X	X
Carbohydrates							X
Tagged proteins						X

List of SPRi-Biochips available with pre-functionalized surfaces

“ready-to-use” SPRi-Biochip surfaces

	Immobilization by direct coupling	Packages
CS	via probes amino group	3, 6 or 9 units
CO	via probes amino group	3, 6 or 9 units
CPe	via biotinylated probes on extravidin surface	3, 6 or 9 units
CSe	via biotinylated probes on extravidin surface	3, 6 or 9 units
COe	via biotinylated probes on extravidin surface	3, 6 or 9 units
CTg	GST-tagged probes on anti-GST antibody derivatized surface CTg	3, 6 or 9 units

“ready-to-use” SPRi-Slide surface

	Immobilization by direct coupling	Packages
CS	via probes amino group	3, 6 or 9 units
CO	via probes amino group	3, 6 or 9 units
CPe	via biotinylated probes on extravidin surface	3, 6 or 9 units
CSe	via biotinylated probes on extravidin surface	3, 6 or 9 units
COe	via biotinylated probes on extravidin surface	3, 6 or 9 units
CTg	GST-tagged probes on anti-GST antibody derivatized surface CTg	3, 6 or 9 units

"we do it for you” SPRi-Biochip surface

	Immobilization by direct coupling	Packages
CPY	the immobilization of pyrrole-conjugate¹ interaction partner with polypyrrole copolymerization on biochip surface	3, 6 or 9 units
CDZ	diazonium salts based procedure for the immobilization of aryl diazonium-derivatives² on the gold chip CDZ	3, 6 or 9 units

“we do it for you” SPRi-Slide surface

	Immobilization by direct coupling	Packages
CPY	the immobilization of pyrrole-conjugate¹ interaction partner with polypyrrole copolymerization on biochip surface	3, 6 or 9 units
CDZ	diazonium salts based procedure for the immobilization of aryl diazonium-derivatives² on the gold chip CDZ	3, 6 or 9 units

¹ Pyrrolated-conjugates are prepared using pyrrole-NHS, pyrrole-phosphoramidite, pyrrole-hydrazide, commercially available (Eras labo). We also provide the conjugates preparation and electrodeposition service. This is available upon request.

² Aryl diazonium-derivatives preparation procedure is published. We also provide the derivatives preparation and electrodeposition service. This is available upon request.

Unfunctionalised surfaces

Au-coated biochips are also available to allow customers to use a free coating technique

		Packages
SPRi-Biochip	Prism with a thin gold layer	5 or 10 units
SPRi-Slide	Slide with a thin gold layer	5 or 10 units
Prism	High index glass prism	2, 5 or 10 units
Index matching liquid	High index liquid for connection between glass prism and SPRi-slides	1, 2 or 3 x 1ml