Applications

# Quantitative Determination of Chlorophyll A with the Duetta Dual Fluorescence/Absorbance Spectrometer

Figure 1: Absorbance spectrum of ISS before and after acidification measured with the Duetta

HORIBA’s Duetta, a CCD-based dual fluorescence/absorbance spectrometer is a very capable instrument for the determination of Chl A concentration by fluorescence due to its inherent sensitivity and ability to automatically correct for the inner filter effect (IFE), which ensures a very high dynamic range for such analysis. It also can be used to quantify the concentration of the standard stock solution by absorbance, thus eliminating a need for a dedicated UV-VIS spectrometer.