HORIBA Scientific decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of instrument response function (IRF, or "lamp" profile) allow accurate recovery of multiple decay components even when the decay data is grossly distorted by the IRF.

DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing for fine-tuning of parameters where necessary.  Analysis modules include reconvolution analysis of multi-exponential models plus analysis of more specialised fluorescence decay processes as detailed below.

Multi-Exponential

Analysis for up to 5 exponentials.

Batch Exponential

Batch mode supports datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring.  It features full reconvolution with up to five exponentials and shift iteration.

Global Exponential

Perform global analyses of up to 5 exponential components and 100 decay curves.

Distribution

Two methods are available for performing lifetime distribution analysis.  The first method employs a “Top Hat” distribution function which allows a single lifetime distribution to be fitted in conjunction with an additional discrete lifetime.  The second method is an optional module which will perform a Non-Extensive Decay distribution which allows up to five lifetime distributions to be modelled.

Exponential Series

Exponential series analysis of up to 30 terms.  This allows parameterisation of otherwise difficult data.

Foerster Energy Transfer

2D and 3D Foerster type energy transfer with an optional exponential component.

Yokoto-Tanimoto Energy Transfer

Yokoto-Tanimoto energy transfer taking into account diffusion with an optional exponential component.

Micellar quenching

Micellar quenching kinetics can help determine the surfactant aggregation number.

Exciplex

Exciplex analysis follows formation of an excited state species.

Anisotropy analysis

Impulse reconvolution of up to 2 correlation times and 5 fluorescence decay components.  Each model parameter, including shift, can be fixed at a pre-determined value or optimised as part of the parameter fitting.  The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse.