Aqualog - A-TEEM Industrial QC/QA Analyzer

Aqualog A-TEEM, Industrial QC/QA Analyzer
EEM Acquisition Speed Comparison

A Simple, Fast, “Column Free” Molecular Fingerprinting Technology

A-TEEM™ Molecular Fingerprinting is a new optical technique that is ideal for comprehensive component analysis in a variety QC/QA applications, from water to wine to pharmaceuticals and more. It is a simple, fast, “column free” spectroscopic technique that simultaneously measures the absorbance, transmission and fluorescence of samples in solution, and offers unique benefits over traditional analytical techniques, such as chromatography, mass spec, IR, etc.. Fundamentally, this technique is best applied to samples in solution that have fluorescing components, like an aromatic molecule, although it also detects non-fluorescing species via absorbance. The fluorescence EEM fingerprint technique has also been applied to solids and skin. Fluorescence is a highly sensitive technique compared to other spectroscopic techniques such as absorbance, FTIR and Raman, and the three-dimensional nature of the data provides for a better foundation for complex component analysis.

A-TEEM spectroscopy refers to the ability to simultaneously acquire Absorbance, Transmittance and a fluorescence Excitation Emission Matrix (A-TEEM) of a particular sample. HORIBA pioneered this technique with the patented Aqualog system, which combines A-TEEM spectroscopy with multichannel CCD detection to provide extremely fast results.

The A-TEEM technique requires no sample preparation, beyond possibly a simple dilution, and the cost and ease of use of an A-TEEM spectrometer allows it to be used both in the research lab as well as at-line or in-line in a production process. It’s a simpler, faster, and cheaper column-free chromatography technique for fluorescence components in solution. A-TEEM has taken fluorescence spectroscopy beyond the research lab and into the analytical industrial QC/QA environment.

HORIBA A-TEEM spectrometers can be used for fluorescence EEMs and for absorbance measurements for multi-component analysis and for transmission measurements for CIE colorimetry.

ASTM D8431 Standard Test Method for Detection of Water-soluble Petroleum Oils (BTEX) by A-TEEM Optical Spectroscopy and Multivariate Analysis.


   Science in Action Series   Spectroscopy Matters Series

Segment: Scientific
Manufacturing Company: HORIBA Scientific

Featured Videos

A-TEEM™ Molecular Fingerprinting Video

Aqualog Data Stream and Sipper Video

Spectroscopy in :60 - FAST -01 Autosampler

EEM Acquisition Speed Comparison Video


  • Compact optical spectrometer based on simultaneous Absorbance-Transmission fluorescence Excitation Emission Matrix (A-TEEMTM) spectroscopy
  • A column free chromatograph that is much faster and easier to operate than traditional analytical techniques
  • Provides inter and intra-lab comparability with NIST traceable corrected spectra
  • Much better sensitivity than IR or Raman spectroscopy (ppm/ppb range depending on the fluorescent molecule)
  • US FDA 21 CFR Part 11 compliant



  • Optimized experiment set-up menus minimize user configuration time
  • Methods and batch protocols for automating multiple sample measurement
  • Easy connectivity to third party chemometric analysis software such as Solo_Predictor from Eigenvector Research Inc.
  • HORIBA Multi-Model Predictor dashboard for enhanced industrial applications
  • Automated titrator accessory
  • Automated sipper accessory


NEW HORIBA Multi-Model Predictor Tool

HORIBA Instruments is pleased to introduce a new software tool designed to automate multivariate and machine learning analysis workflows for industrial QC/QA applications of HORIBA’s patented Aqualog Absorbance- Transmission fluorescence Excitation Emission Matrix (A-TEEM™) spectrometer.

Key Applications of HMMP Tool

  • Wine and Grape Quality Chemistry (phenolics, anthocyanins, tannins, sulfites etc..)
  • Water Contamination (oil, algae and other materials)
  • Grain mold and odor compounds
  • Cannabinoids
  • Pharmaceuticals (Drugs, Vaccines, Cell Media)
  • Olive Oil adulteration and spoilage
  • Dietary supplement adulteration


Key Features and Benefits

  • Easy, Rapid Operator Level Analysis
  • Facilitated Administration of Method Model Development and Editing
  • Complete Parameter Profile and Classification Reports
  • Compatible with Laboratory Information Management Systems
  • HMMP Add-In Fully Integrated into Eigenvector Inc. Solo/Solo+Mia and Exclusively Activated and Supported by HORIBA Instruments Inc.


NEW A-TEEM™ technology

A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!

All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.

Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.

NEW Automatic Sipper Accessory

New for our Aqualog®  A-TEEM™ spectrometer, the Aqualog automatic sipper accessory handles sampling from a single source, in addition to dispensing rinsing solutions, detergents and controlling reverse-flow drainage The 4-sample changer unitconnects to the main sipper unit to enable sampling of up to 4 sources.

The sipper offers convenient installation and operation, with built-in automatic cleaning, leak detection and protection.  It is fully integrated into the new Aqualog 4.0 software for batch analysis and has a variety of uses in water, pharmaceutical, beverage phenolics and many other applications.

When used at a water treatment plant, the sipper and four channel accessory enable the Aqualog to automatically extract and monitor raw, settled and finished water samples. Each sample changer unit is compatible with overflow and filtration devices, serving up to 4 independent water treatment plant sources..

NEW Fast-01 Autosampler Accessory

The Fast-01 can be configured to use a variety of sample-vials and racks to meet your application needs and enables complete temperature control. Sample vial repeats and injection volumes are easily facilitated with the Aqualog 4.2+ software, which also offers preconfigured blank files. 

All data files can be exported with ISO-formatted time-date stamping and user-configurable Sample ID and repeat codes.

All aspects of the Fast-01 hardware control are at your fingertips, with key real-time access features to facilitate the configuration and execution of your batch experiments, as well as priming, cleaning and maintenance.

Fluorescence Hardware

Light source

Extended-UV: 150W vertically mounted xenon arc lamp

Excitation range

200 nm to upper limit of emission detector

Excitation bandpass

5 nm

Excitation monochromator

Subtractive double monochromator

Excitation gratings

1200 gr/mm, 250 nm blaze

Excitation wavelength accuracy

±1 nm

Choice of Detector


Emission range

250-800 nm

Emission grating

285 gr/mm; 350 nm blaze

Hardware pixel binning

0.58, 1.16, 2.32, 4.64 nm/pixel

Emission bandpass

5 nm

Emission spectrograph

Fixed, aberration-corrected 140 mm focal length

Emission detector

TE-cooled back-illuminated CCD

Emission integration time

5 ms minimum

CCD gain options

2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain


Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)


32.72 kg (72 lbs)


LWH (618 x 435 x 336 mm); (24" x 17" x 13")

Absorbance Hardware

Scanning range

200-800 nm (UV lamp)


5 nm

Slew speed

Maximum 500 nm/s
Optical systemCorrected single-beam
DetectorSi photodiode

Wavelength accuracy

+/- 1 nm

Wavelength repeatability

+/- 0.5

Photometric accuracy

+/- 0.01 AU from 0 to 2 AU
Photometric stability<0.002 AU per hour
Photometric repeatability+/- 0.002 AU (0 to 1 AU)

Stray light

<1% measure with Kl standard

Quantitative Chemometric Modeling of Kavalactones and Flavokavains in Kava Root with A-TEEM™ Fluorescence Spectroscopy
Quantitative Chemometric Modeling of Kavalactones and Flavokavains in Kava Root with A-TEEM™ Fluorescence Spectroscopy
In this application note, a method for accurately predicting the amounts of major constituents of kava root from spectroscopic absorbance and fluorescence measurements made with the HORIBA Aqualog® and the A-TEEM technique is presented.
A-TEEM Fluorescence Spectroscopy for the Quick Detection of Adulteration in Lavender Essential Oils
A-TEEM Fluorescence Spectroscopy for the Quick Detection of Adulteration in Lavender Essential Oils
Verifying the quality of lavender essential oil is crucial to ensure it is safe for its intended use, and to ensure consumers aren’t being deceived into buying inferior products. Two samples of lavender essential oil with identical label claims of "Pure", one from a supplier that tests for quality and one that does not, were evaluated with A-TEEM spectroscopy and GC-MS.
Trihalomethane Speciation Modeling in Drinking Water Using the A-TEEM Spectroscopic Technique and Multivariate Data Analysis
Trihalomethane Speciation Modeling in Drinking Water Using the A-TEEM Spectroscopic Technique and Multivariate Data Analysis
This application note describes a rapid, sensitive nondestructive disinfection byproducts (DBP) prediction method using patented simultaneous Absorbance-Transmittance and fluorescence Excitation-Emission Matrix (A-TEEM) spectroscopy.
Monitoring Biotinylated Adeno-Associated Virus (AAV) using A-TEEM
Monitoring Biotinylated Adeno-Associated Virus (AAV) using A-TEEM
Adeno-Associated Viruses (AAVs) are becoming widely used as viral vectors to deliver cell and gene therapies and vaccines. As production methods scale to meet growing demand, more information is needed more quickly.
Monitoring Cell Culture Media Variability using a Simple Optical Technique (A-TEEM Molecular Fingerprinting)
Monitoring Cell Culture Media Variability using a Simple Optical Technique (A-TEEM Molecular Fingerprinting)
With the rise of protein production using mammalian cell culture, it has become increasingly important to control the quality of the cell culture media for use in production processes. Cell culture media are usually prepared as aqueous solutions and should provide everything a cell line needs for optimal cell growth as well as product yield and quality.
Identification and Quantification of Potential Adulterants in Cranberry Fruit Juice Dry Extracts using Absorbance-Transmittance Excitation-Emission (A-TEEM) Spectroscopy
Identification and Quantification of Potential Adulterants in Cranberry Fruit Juice Dry Extracts using Absorbance-Transmittance Excitation-Emission (A-TEEM) Spectroscopy
The Aqualog A-TEEM spectrometer has the capacity to specifically identify and sensitively quantify key adulterants that are most commonly detected in Cranberry dietary supplements, including Pine Bark, Grape Seed and Peanut Skin extracts.
Insulin Structure and Stability Assessment
Insulin Structure and Stability Assessment
Stability and aggregation of insulin are studied using simultaneous fluorescence excitation emission matrices (EEMs) and UV-Vis absorbance spectroscopy [1]. Insulin is a protein-hormone, produced by the pancreas and is necessary for basic metabolic processes. The different types of commercial insulin therapeutics generally fall into two categories: short-acting and long-acting insulin. The difference between some short-acting and long-acting insulin is, in some cases, only one to three residues in the protein sequence.
Spectroscopic Analysis of Red Wines with Aqualog
Spectroscopic Analysis of Red Wines with Aqualog
HORIBA’s Aqualog is uniquely equipped with patented simultaneous Absorbance spectral and fluorescence excitation-emission matrix (EEM) technology which provides rapid access to a wide range of parameters important to commercial wine processing and quality characterization.
Classification and Phenolics Analysis of Red Wines with A-TEEM Molecular Fingerprinting
Classification and Phenolics Analysis of Red Wines with A-TEEM Molecular Fingerprinting
Aqualog®, an analytical instrument based on the simultaneous measurement of Absorbance, Transmission, Fluorescence Excitation and Emission Matrix (A-TEEM). A-TEEM fingerprints yield qualitative and quantitative composition of key flavor and color determinants in wine and spirits that are not discernible with simple Absorbance or Transmission data analysis.
Spectroscopic Methods for Sunscreens Characterization
Spectroscopic Methods for Sunscreens Characterization
This Application Note outlines three different kinds of spectroscopic tools being used for the characterization of sunscreens, and discusses the obtained results. These include Fluorescence spectroscopy for photoactivity, Particle Size analysis for composition and Raman microscopy for formulation investigation.
Rapid Extra Virgin Olive Oil Classification and Blend Quantitation
Rapid Extra Virgin Olive Oil Classification and Blend Quantitation
The resurgence of interest in the Mediterranean Diet and its associated health benefits have directed focus on the role that Extra Virgin Olive Oil plays. The increasing awareness is leading to increasing product demand, but also opportunities to compromise quality. Hence the need for rapid analytical methods to perform Quality Analysis of various product samples.

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