Fluorescence spectroscopy is widely used in various industries, including life sciences. Two-dimensional (2D) fluorescence excitation-emission matrix (EEM) spectroscopy has been utilized for over 30 years to characterize or “fingerprint” biological samples.

随着利用哺乳动物细胞培养生产蛋白质的兴起，在生产过程中控制细胞培养基的质量变得越来越重要。用于生物反应器的细胞培养基通常为水溶液，并提供细胞最优生长所需的一切物质。

监测培养基的条件对促进再生药物和蛋白质合成等过程中的细胞增殖非常重要。由于培养基介质组份复杂，且荧光成份多样，因此需要荧光激发发射三维矩阵(EEM)来同时全面展现发光信息。本篇报告主要通过测量EEM来监测细胞增殖过程中的细胞培养基组份的变化。

众所周知，胰岛素是一种由胰腺产生的蛋白质激素，是基本代谢过程中所必需的激素。对于胰岛素的稳定性和聚集性等性能检测显得尤为重要，已有文献报道了通过荧光激发发射矩阵(EEMs)和紫外-可见吸收光谱（UV-Vis）来研究。[1]目前，商业治疗胰岛素主要分为两类: 速效胰岛素和长效胰岛素。相比于长效胰岛素，速效胰岛素在蛋白质序列中只有1到3个残基。

In this paper, HORIBA presents the Centre for Process Innovation (CPI) evaluation work of A-TEEM as a suitable technique to analyse six in-process purification samples of monoclonal antibodies—an initial step toward implementing this technique as a PAT solution in biologics manufacturing.

Accurate monitoring of bioprocesses is essential, as small variations in culture conditions can impact productivity, product quality, and process consistency. One key challenge lies in analyzing the complex, dynamic environment of bioreactors, where media composition, metabolic byproducts, and matrix effects introduce variability. As the industry shifts toward intensified and continuous processing, there's growing demand for rapid, real-time analytical tools that operate under sterile, GMP-compatible conditions. A-TEEM™ technology meets these requirements with its high sensitivity, molecular selectivity, and real-time data output, providing actionable insights for optimizing cell growth and overall bioprocess performance.

Traditional methods for assessing water quality, like chemical oxygen demand (COD) and total organic carbon (TOC) analysis, are often time-consuming and costly. This application note details a more efficient and cost-effective approach using optical spectroscopic techniques to characterize dissolved organic matter (DOM) and monitor anthropogenic organic pollutants from aquafarms.