Rapid, Label-Free Protein and Peptide Quantification with Sub-ppm LOQ Using A-TEEM™ Spectroscopy

Accurate protein and peptide quantification underpins critical decisions in biopharmaceutical R&D and quality control, yet routine methods often suffer from matrix effects and limited structural insight.

Absorbance-Transmittance Excitation-Emission Matrix (A-TEEM) spectroscopy integrates UV–Vis absorbance with full fluorescence EEM data in a single, rapid, label-free measurement. This application note demonstrates how A-TEEM delivers accurate, highly linear, and reproducible protein quantification with sub-ppm limits of detection and quantification. In addition to sensitive concentration measurements, A-TEEM simultaneously generates rich spectral fingerprints, providing valuable structural and compositional insight from a single rapid, label-free analysis.

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