Dr Wei Huang is an Associate Professor at University of Oxford and EPSRC research fellow.  He got a PhD from University of Sheffield in 2002.  He was a senior research fellow, lecturer and senior lecture 2007-2014 in University of Sheffield.  From 2002-2007, he has worked as a postdoctoral associate at University of Wales and Oxford.  His research interests are Raman single cell biotechnology and synthetic biology.  He has published more than 60 peer-reviewed journal papers.  He is internationally recognised for his pioneering work in developing single cell Raman biotechnology and leads this field after publishing the first paper in 2004.  He acted as a guest editor to address single cell biotechnology for Current Opinion in Biotechnology in 2012.

会议报告主题/Topic:
Progress of Raman sorting for single cell research

摘要/Abstract:
Single cell biology is a high resolution biology which will open a new frontier for understanding the basic functional unit of life and usher potential application to cancer diagnosis and new gene discovery. Single cell Raman spectra (SCRS) can be regarded as a phenotypic fingerprint of individual cells which can be used to characterise cell type, physiological state and cell functionalities. To date, we have developed Raman activated cell sorting (RACS) and Raman activated cell ejection (RACE), which sort cells according to their Raman spectra RACS and RACE have been applied to in seawater or soil/sludge samples. RACS and RACE will revolutionise our ability to study single cells in their native states and pave the way for single cell ‘-omics’ including genomics, transcriptomics, proteomics and metabolomics.
 
Reference:

  • Berry et al. (2015) Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells. Proc Natl Acad Sci U S A 112:E194-203.
  • Zhang et al. (2015) Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community. ISME J 9:603-614.
  • Zhang et al. (2015) Raman-activated cell sorting based on dielectrophoretic single-cell trap and release. Anal Chem 87:2282-2289.
  • Wang et al. (2013) Rapid Raman Activated Cell Ejection for Isolation of Single Cells. Anal Chem. 85:10697-10701.
  • Li et al. (2013) Stable isotope probing and Raman spectroscopy for monitoring carbon flow in a food chain and revealing metabolic pathway. Anal Chem 85: 1642-1649.
  • Li et al. (2012) Rapid Resonance Raman micro-spectroscopy to probe carbon dioxide fixation by single cells in microbial communities. ISME J 6:875-885.
  • Huang et al. (2007) Raman-FISH: Combining stable-isotope Raman spectroscopy and fluorescence in situ hybridization for the single cell analysis of identity and function. Environ Microbiol 9: 1878-1889.
  • Huang et al. (2004) Raman microscopic analysis of single microbial cells. Anal Chem 76: 4452-4458.